A light source unit (3) for simultaneously irradiating the object (X) with illumination light (Lw) and excitation light (Lex) having a partial wavelength band out of the illumination light wavelength band A single imaging device (52) for simultaneously photographing reflected light (Lw ) from the object (X) and fluorescence (Lf) generated in the subject (X) by irradiation of the excitation light (Lex) A filter (53) for transmitting the light (Lw , Lf) excluding the excitation light (Lex) to the imaging device (52) And a light control unit (64) for adjusting the intensity independently of each other.照明光(Lw)と、該照明光の波長帯域のうち一部の波長帯域を有する励起光(Lex)とを同時に被写体(X)に照射する光源部(3)と、照明光(Lw)の被写体(X)からの反射光(Lw’)と、励起光(Lex)の照射によって被写体(X)において発生する蛍光(Lf)とを同時に撮影する単一の撮像素子(52)と、励起光(Lex)を除く光(Lw’,Lf)を撮像素子(52)へ透過させるフィルタ(53)と、光源部(3)からの照明光(Lw)の出力強度と励起光(Lex)の出力強度とを互いに独立に調整する調光部(64)とを備える蛍光観察装置(100)を提供する。
