With is capable of emitting excitation light of a plurality of wavelength bands are different from each other, the fluorescence observation apparatus of the present invention, a plurality of excitation light to be emitted on the subject of the second phosphor, and the first phosphor is present a light detector for detecting a variable filter unit that selectively transmits at least a portion of the wavelength band light source unit can be selected from the excitation light, return light from the subject is provided, the light transmitted through the variable filter part, Based on the strength properties of the excitation light, a plurality of fluorescence properties of the phosphor of the first and on the fluorescence properties of the second phosphor, the transmission wavelength band of the variable filter part and the wavelength band of the excitation light emitted from the light source and includes a processing unit for performing operations to respectively determine, and a control unit based on the calculation result of the arithmetic processing unit, performs control to synchronize a variable filter part and the light source unit.本発明の蛍光観察装置は、波長帯域が相互に異なる複数の励起光を出射可能であるとともに、第1の蛍光体及び第2の蛍光体が存在する被写体に対して出射する励起光を複数の励起光から選択可能な光源部と、被写体からの戻り光が具備する波長帯域の少なくとも一部を選択的に透過させる可変フィルタ部と、可変フィルタ部を透過した光を検出する光検出部と、複数の励起光の強度特性と、第1の蛍光体の蛍光特性と、第2の蛍光体の蛍光特性と、に基づき、光源部から発せられる励起光の波長帯域及び可変フィルタ部における透過波長帯域をそれぞれ決定するための演算を行う演算処理部と、演算処理部の演算結果に基づき、光源部と可変フィルタ部とを連動させる制御を行う制御部と、を有する。
