The invention provides means and methods for interfering with Programmed Cell Death 1 protein (PD-1) and T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) mediated inhibition in a PD-1 and/or TIM-3 positive cell. A method may comprise contacting said cell with an antibody or a functional part, derivative and/or analogue thereof that comprises a variable domain that can bind to an extracellular part of PD-1 and a variable domain that can bind to an extracellular part of TIM-3, thereby inhibiting PD-1 and/or TIM-3 mediated activity in said cell. The invention also provides antibodies or variants thereof that comprises a variable domain that can bind to an extracellular part of PD-1 and a variable domain that can bind to an extracellular part of TIM-3.本發明提供，在計劃性細胞死亡1蛋白質(Programmed Cell Death 1 protein，PD-1)和/或T細胞免疫球蛋白質及含有黏蛋白質域-3(T-cell immunoglobulin and mucin-domain containing-3，TIM-3)陽性細胞中，干擾PD-1和TIM-3所介導的抑制的手段和方法。一種方法可包含使所述細胞與抗體或其功能部分、衍生物和/或類似物接觸，其中前述抗體或其功能部分、衍生物和/或類似物包含可結合至PD-1的胞外部分的可變域和可結合至TIM-3的胞外部分的可變域，藉此抑制所述細胞中由PD-1和/或TIM-3所介導的活性。本發明亦提供包含可結合至PD-1的胞外部分的可變域和可結合至TIM-3的胞外部分的可變域的抗體或其變異體。
