MÉTHODES ET COMPOSITIONS POUR LA MODIFICATION D'ADN CIBLE

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专利权人:

The Regents of the University of California

发明人:

申请号：

EP14811149.5

公开号：

EP3008181A2

申请日:

2014.06.10

申请国别(地区):

EP

年份:

2016

代理人:

摘要：

The disclosure provides compositions and methods for increasing efficiency of Cas9-mediated target DNA modification. Specifically, the disclosure provides compositions and methods for carrying out site-directed modification of a target DNA, the methods comprising contacting the target DNA with: a) a complex comprising a Cas9 polypeptide and a guide RNA, and b) a Rad51 polypeptide. The site-directed modification of a target DNA can be carried out in a living cell in vitro, in a living cell in vivo, or in a cell-free system in vitro.La présente invention concerne des compositions et des méthodes qui permettent d'augmenter l'efficacité de modification d'ADN cible à médiation assurée par Cas9.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/