The present invention provides a method for producing glucose from a lignocellulosic feedstock. The method comprises the steps of (i) providing a pretreated lignocellulosic feedstock slurry； (ii) moving the pretreated feedstock slurry through a pipe, which slurry has an undissolved solids content of between about 15 and about 30 wt%； (iii) adding cellulase enzyme tothe pretreated slurry to produce a slurry comprising cellulase enzyme； (iv) dispersing the cellulase enzyme added in step (iii) in the pretreated slurry by using a high shear in-line mixing device, thereby producing a pretreated slurry comprising dispersed cellulase enzyme； and thereafter (v) subjecting the pretreated slurry comprising dispersed cellulase enzyme to hydrolysis so as to produce glucose from cellulose contained therein.
