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METHOD FOR PRODUCING LIPID PARTICLE AND NUCLEIC ACID DELIVERY CARRIER COMPRISING LIPID PARTICLE
专利权人:
Fujifilm Corporation
发明人:
NISHIKAWA, Naoyuki,SUGIYAMA, Susumu,SEKIGUCHI, Takahiro,ONO, Makoto
申请号:
EP15822112
公开号:
EP3170504A4
申请日:
2015.07.16
申请国别(地区):
EP
年份:
2017
代理人:
摘要:
An object of the present invention is to provide a method for producing lipid particles which can hold nucleic acids or the like at a high inclusion rate, and a nucleic acid delivery carrier having the lipid particles. According to the present invention, there is provided a method for producing lipid particles including nucleic acids, the method including the following steps of (1) to (4): (1) a step of heating an oil phase containing a phospholipid, an alcohol, and an ester (2) a step of mixing an oil phase prepared in step (1) with a water phase containing nucleic acids (3) a step of cooling the mixed liquid which contains the oil phase and the water phase and has been obtained in step (2), and crystallizing lipid particles and (4) a step of removing the alcohol and the ester from the mixed liquid which contains the oil phase and the water phase and has been obtained in step (3).
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