Naoyuki NISHIKAWA,Susumu SUGIYAMA,Takahiro SEKIGUCHI,Makoto ONO
申请号：
US15405983
公开号：
US20170121714A1
申请日:
2017.01.13
申请国别(地区):
US
年份:
2017
代理人:
摘要：
An object of the present invention is to provide a method for producing lipid particles which can hold nucleic acids or the like at a high inclusion rate, and a nucleic acid delivery carrier having the lipid particles. According to the present invention, there is provided a method for producing lipid particles including nucleic acids, the method including the following steps of (1) to (4): (1) a step of heating an oil phase containing a phospholipid, an alcohol, and an ester (2) a step of mixing an oil phase prepared in step (1) with a water phase containing nucleic acids (3) a step of cooling the mixed liquid which contains the oil phase and the water phase and has been obtained in step (2), and crystallizing lipid particles and (4) a step of removing the alcohol and the ester from the mixed liquid Which contains the oil phase and the water phase and has been obtained in step (3).
