PROBLEM TO BE SOLVED: To provide a manufacturing method of lipid particles which is capable of holding nucleic acid or the like at a high inclusion ratio, and a nucleic acid delivery carrier having lipid particles.SOLUTION: A manufacturing method of lipid particles including nucleic acid includes processes (1) to (4) below: (1) a process of heating oil phase containing phospholipid, alcohol and ester (2) a process of mixing water phase containing nucleic acid and the oil phase prepared in process (1) (3) a process of cooling liquid mixture containing the oil phase and the water phase obtained in process (2) to crystallize lipid particles and (4) a process of removing alcohol and ester from the liquid mixture containing the oil phase and the water phase obtained in process (3).SELECTED DRAWING: NoneCOPYRIGHT: (C)2016,JPO&INPIT
