To provide a production method of a composition derived from banana capable of acquiring a composition exhibiting an excellent bioactivity from a pulp part of banana.SOLUTION: There is provided a production method of a composition derived from banana comprising: a step (1) for fragmenting a pulp part of banana for acquiring a pulp fragmentation matter; a step (2) for adding a protein degrading enzyme for decomposing a protein in the pulp fragmentation matter by an enzyme reaction, for acquiring an enzyme reaction matter; and a step (3) for deactivating the protein degrading enzyme in the enzyme reaction matter, for acquiring a composition A.SELECTED DRAWING: Figure 1【課題】バナナの果肉部から、優れた生理活性を奏する組成物を得ることができる、バナナ由来組成物の製造方法の提供。【解決手段】バナナの果肉部を細分化し、果肉細分化物を得る工程(1)と、前記果肉細分化物に、蛋白質分解酵素を添加して酵素反応により前記果肉細分化物中の蛋白質を分解し、酵素反応物を得る工程(2)と、前記酵素反応物中の前記蛋白質分解酵素を失活させ、組成物Aを得る工程(3)と、を備える、バナナ由来組成物の製造方法。【選択図】図1