PURPOSE: A cultivating method of fruits and vegetables is provided to manufacture chitooligosaccharide which is low-molecularized to a condition that fruits and vegetables can be absorbed by being resolved to an oligomer form in a short reaction time. CONSTITUTION: A cultivating method of fruits and vegetables comprises the steps of: mixing a fixed amount of 0.1-3% chitosan aqueous solution comprising chitosan having a molecular weight of over 200,000 (Mw) with a fixed amount of chitosanase strain culture； culturing the chitosanase by keeping the mixture for 1-4 hours； putting the mixture containing the cultured chitosanase into a mixer after filling up the mixer with the 0.1-3% chitosan aqueous solution； mixing the mixture in a mixer at 500-1500 rpm for 5-15 minutes； mixing the mixture at 100-300 rpm for 2-10 hours； and injecting the produced chitooligosaccharide to fruits and vegetables. The chitonase is a strain among Rhizopus sp., Streptomyces griseus, Bacillus sp., Trichoderma hamatum, Trichoderma harzianum, Pseudomonas putida, and Pseudomonas aeruginosa. [Reference numerals] (S1) Mix a chitosanase strain culture in a chitosan aqueous solution； (S2) Culture chitosanase for 1-4 hours； (S3) Fill the chitosan aqueous solution in a stirrer and add the chitosanase； (S4) Stir at a high speed of 500-1500 rpm； (S5) Stir at a low speed of 100-300 rpm； (S5') Inject air bubbles； (S6) Add chitosan oligosaccharides to fruits and vegetables본 발명은 키토올리고당 발효액비를 이용한 과채류의 재배방법에 관한 발명으로서, 보다 상세하게는 분자량 200,000 이상의 키토산을 라이조푸스 균주 배양물을 발효시켜 분자량 10,000 이하의 올리고머 형태로 분해시켜 이를 액비로 과채류에 관주, 또는 엽면시비 한 결과, 해당 과채류에서 키토올리고당이 과채류 체성분으로 검출되어 특정한 기능성을 가지는 과채류 생산이 가능하게 되는 키토올리고당 발효액비를 이용한 과채류의 재배방법에 관한 발명이다.
