PROBLEM TO BE SOLVED: To provide a method that makes it possible to efficiently and stably produce casein hydrolysate comprising a peptide comprising a Lys-Pro-Val sequence in the state of a tripeptide.SOLUTION: A method of producing casein hydrolysate comprises decomposing casein using an enzyme derived from genus Aspergillus microorganisms, and pancreatin, thereby producing casein hydrolysate comprising a peptide comprising a Lys-Pro-Val sequence in the state of a tripeptide.