Provided in the present invention is a manufacturing method of a sea cucumber extract, which comprises the steps of: (1) adding protamex to sea cucumber powder and enzymatically degrading the same； (2) filtering the enzymatically degraded product obtained from step (1)； and (3) freeze-drying a filtrate obtained from step (2). According to the present invention, the sea cucumber extract with abundant bioactive materials at high yields can be obtained through a simple and inexpensive method.본 발명은 (1) 해삼 분말에 프로타멕스(protamex)를 첨가하여 효소분해하는 단계； (2) 상기 단계 (1)에 의해 얻어진 효소분해물을 여과하는 단계； 및 (3) 상기 단계 (2)에 의해 얻어진 여과액을 동결건조하는 단계；를 포함하는 해삼추출물의 제조방법을 제공한다.
