Provided in the present invention is a manufacturing method of a sea cucumber extract, which comprises the steps of: (1) mixing water with ethanol, and manufacturing 60-70% of ethanol； (2) performing sonication in the time of one to five hours after inserting 60-70% of ethanol in a sea cucumber powder sample, and covering an entrance with a foil； (3) extracting the same for 6 to 24 hours and then filtering the same； and (4) freeze-drying the filtered extract. According to the present invention, the sea cucumber extract with abundant bioactive materials at high yields can be obtained through a simple and inexpensive method.본 발명은 (1) 에탄올과 물을 혼합하여 60~70% 에탄올을 제조하는 단계； (2) 해삼 분말시료에 60~70% 에탄올을 넣어준 뒤, 호일로 입구를 감싼 다음 1~5 시간 동안 시간 동안 소니케이션을 수행하는 단계； (3) 6~24시간 동안 추출한 후 여과하는 단계； 및 (4) 여과된 추출물을 동결건조하는 단계를 포함하는 해삼추출물의 제조방법을 제공한다.
