The present invention provides improved and/or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. The methods may comprise gene-editing at least a portion of the TILs to enhance their therapeutic efficacy. Such TILs find use in therapeutic treatment regimens.本發明提供用於擴增TIL及產生TIL治療性族群之改善及/或縮短方法，包括在密閉系統中擴增TIL族群之新穎方法，該方法導致在較短期間內改善TIL的療效和表型且增加代謝健康，同時允許減少微生物污染也降低成本。該方法可包含基因編輯至少一部分的TIL以增強彼等之治療療效。該TIL可用於治療性處置方案(regimen)。
