The present invention provides improved and/or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.本發明提供經改善和/或經時程縮短之用於擴增TIL和製造治療性TIL族群之方法，包括用於在封閉系統中擴增TIL族群之新穎方法，其在較短時程中產生療效改善、性狀改善、和TIL之代謝健康增加，同時考量降低微生物污染與降低成本。此等TIL能用於治療性治療給藥方案。
