A method for manufacturingCordycepsCicadaemycelia active substance for preventing and/or improving acute lung injury is provided. The methodcomprisesfollowing steps: （a）culturing aCordycepsCicadaemycelium in a plate media between 15℃ and 30℃ for 1 to 2 weeks； （b）inoculating the mycelium of step （a） to a flask and culturing it between 15℃ and 30℃ with a pH of 2 to 6 for 3 to 14 days； （c）inoculating the mycelium of step （b） to a fermentation tank and culturing it between 15℃ and 30℃ with a pH of 2 to 6 for 3 to 21 days, so as to obtain aCordycepsCicadaemycelium fermentation liquid containing saidCordycepsCicadaemycelium active substance.本發明提供一種用於預防及/或改善急性肺損傷的蟬花菌絲體活性物質，該活性物質的製備方法，包括下列步驟：（a）取一蟬花菌絲體 （CordycepsCicadaemycelia）於平板培養基上，於15至30℃的溫度下培養1至2周；（b）將步驟（a）培養後的蟬花菌絲體接種至一燒瓶內，於15至30℃、pH 2至6的環境培養3至14天；（c）將步驟（b）培養後的蟬花菌絲體接種於一發酵槽內，於15至30℃、pH 2至6的環境下攪拌培養3至21天，形成含有該蟬花菌絲體活性物質的一蟬花菌絲體發酵液。
