The present invention relates to regulatory factors for FOXP3 and regulatory T cells and application thereof. Specifically, the present invention relates to the use of an ubiquitination pathway-related factor, its agonist or antagonist in the preparation of a composition for regulating FOXP3, IL-2, and/or IFN-γ activity, in which the ubiquitination pathway-related factor is selected from: Toll-like receptor, ubiquitin ligase, pro-inflammatory cytokine family receptor, and/or its coding sequence. The new type of regulatory factors of the present invention can regulate regulatory T cells and immune system by regulating FOXP3, IL-2, and/or IFN-γ activity. The regulatory factors and their derivatives of the present invention can also be used as immunoadjuvant for treating or preventing major diseases (such as, infectious diseases and tumor, etc).
