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Procedures for the production of T lymphocytes and T lymphocytes produced by them
专利权人:
ANTHROGENESIS CORPORATION
发明人:
申请号:
ARP160104094
公开号:
AR107278A1
申请日:
2016.12.30
申请国别(地区):
AR
年份:
2018
代理人:
摘要:
4. The method of producing T cells from peripheral monocytes, for example, there is no single stage to generate and separate antigen display cells, only one round of antigen stimulation. Methods of using these T-cell toxins, such as those for cancer and / or viral infection. 1. Requirement 1: a method of producing a cell population containing specific T antigen cells (e.g. cytotoxic T lymphocytes),characterized in that it comprises the steps of: (a) isolating peripheral blood mononuclear cells (PBMCs) from a subject; (b) culturing said PBMCs in an antigen presenting cell induction medium (APC) comprising interleukin 4 (IL-4) and soluble CD40 ligand (sCD40L) and / or comprising macrophage-granulocyte colony stimulating factor (GM -CSF) and interferon a (IFN-a),(c) The first group of cells is cultured with one or more antigens to produce the second group of cells; and (d) the second group of cells is cultured in a T cell expansion environment including blood cancer 7 (IL-7),interleucina 15 (IL-15)The third group of cells consists of T cells, which are CD3 + type and are specifically used for the third (c) antigen.Métodos para generar células T, por ejemplo, linfocitos T citotóxicos a partir de células mononucleares de sangre periférica sin una etapa separada para generar y aislar células presentadoras de antígenos, y con una sola ronda de estimulación antigénica. También métodos de utilización de dichos linfocitos T citotóxicos, por ejemplo, para tratar cáncer y/o infección viral. Reivindicación 1: Un método para producir una población de células que comprende células T específicas para antígenos, por ejemplo, linfocitos T citotóxicos (CTLs), caracterizado porque comprende las etapas de: (a) aislar células mononucleares de sangre periférica (PBMCs) de un sujeto; (b) cultivar dichas PBMCs en un medio de inducción de células que presentan antígenos (APC) que comprende interleucina 4 (IL-4) y ligando CD40 soluble (sCD40L) y/o que comprende factor estimulador de colonias
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