The present invention provides a lactic acid bacterium suppressing secondary fermentation of silage and being useful for a preferable silage preparation.Another object of the present invention is to provide a method for producing a secondary fermentation-suppressed. As a silage solution means, Lactobacillus diolivorans having an anti-yeast action is used as a lactic acid bacterium for silage preparation.本發明之課題是提供抑制青貯料的二次發酵,對調製理想的青貯料有用的乳酸菌。又本發明之課題是提供製造抑制二次發酵的青貯料的方法。作為解決手段是以具有抗酵母作用的廸氏乳桿菌(Lactobacillus diolivorans)作為青貯料調製用乳酸菌。