PROBLEM TO BE SOLVED: To provide a method for producing fat and oil with high diacylglycerol content by esterification with a fatty acid, and effectively producing the fat and oil containing high amount of highly pure diacylglycerol in an industrially advantageous condition.SOLUTION: The method for producing the fat and oil with high diacylglycerol content includes: reacting glycerine and the fatty acid, or a lower alkyl ester thereof using an immobilized lipase in which a 1,3-selective lipase is immobilized on an immobilized carrier having an average particle size of less than 300 &mum.COPYRIGHT: (C)2012,JPO&INPIT【課題】脂肪酸をグリセリンとエステル化してジアシルグリセロール高含有油脂を製造する方法であって、高純度のジアシルグリセロール高含有油脂を工業的に有利な条件で効率よく製造する方法を提供。【解決手段】平均粒径300μm未満の固定化担体に1,3-位選択リパーゼを固定化した固定化リパーゼを用いて、グリセリンと脂肪酸又はその低級アルキルエステルとを反応させるジアシルグリセロール高含有油脂の製造方法。【選択図】なし
