That when it is applied for the purpose of pasteurizing the present invention relates food products, to develop a new method for membrane permeabilization, which can improve the mortality rate of undesirable cells. The method for the membrane permeabilization of biological cells contained in [SOLUTION] product and releases a pulse electric field is applied to the processing device comprising a process chamber at least one, the next step: - living cells supply a predetermined flow rate products containing and feeding the processing device from the supply unit, which contains the product - feed flow rate of the above plus the extraction flow rate described in the take-out the following steps, the product containing the living cells step is introduced into the processing chamber by introducing a flow rate that corresponds to - treating a pulsed electric field to products that are introduced into the chamber - downstream of the supply unit upstream of the chamber, the product at the outlet of the chamber In order to transport again, including the step of taking out taking out a given flow rate, and method. [Selection] Figure Figure 1【課題】食品製品を低温殺菌するという目的で適用される場合に、所望でない細胞の死亡率を改善できる膜透過化のための新規な方法を開発すること。【解決手段】製品中に含有される生体細胞の膜透過化のための方法であって、パルス電場を放出する、少なくとも1つの処理チャンバを含む処理デバイスに適用され、次の工程:-生体細胞を含む製品を所定の供給流速で、製品を含む供給ユニットから処理デバイスに供給する工程;-この生体細胞を含む製品を、以下の取り出し工程にて記載される取り出し流速を加えた上述の供給流速に対応する導入流速で処理チャンバ中に導入する工程;-このチャンバに導入された製品をパルス電場で処理する工程;-このチャンバの出口における製品を、このチャンバより上流でこの供給ユニットより下流に再び輸送するために、所定の取り出し流速で取り出す工程を含む、方法。【選択図】図1
