The present invention relates to a composition for preventing and treating osteoarthritis, which contains a pomegranate concentrate, a eucommia bark extract and an achyranthis radix extract as active ingredients. More specifically, the composite composition of a pomegranate concentrate, a eucommia bark extract and an achyranthis radix extract according to the present invention has a synergistic effect of improving osteoarthritis. In particular, because it can directly protect and regenerate the cartilage and can improve joint stiffness, it can be used in health food, etc.