PURPOSE: A culture medium composition containing gold ions is provided to be used for cultivating all kinds of mushrooms.CONSTITUTION: A culture medium is manufactured by the following steps: mixing 30 wt% scattered straws (or peel of soybean, sawdust of black oak, sawdust of poplar tree, sawdust of Douglas fir, sawdust of poplar tree), 10 wt% bean-curd dregs, 15 wt% rice bran, 5 wt% dried poultry waste powder, 3 wt% calcium hydroxide powder, 2 wt% plaster powder, 5 wt% cotton, 5 wt% arrow-root root powder, 15 wt% microalgae powder, 15 wt% coffee sediment powder, 5 wt% residue of herb medicine powder diluting 1-10ppm of 20-35nano colloidal gold having concentration of 1000ppm with water 65 wt% of the culture medium weight to make the concentration be 1-10ppm aging the mixture by dispersing and mixing at intervals of two days for 15 days and sterilizing the mixture with steam at 100-120°C for 5-10 hours. A cultivation method using the culture medium comprises the following steps: filling up a plastic bottle or plastic bag with about 700gm of the manufactured culture media and injecting mushroom starters culturing the starters at 20-21°C in 67% humidity for 15-35 days growing at 14-15°C in 85% humidity after germinating at 16-17°C in 95% humidity for 10 days. The mushrooms include pleurotus eryngii, lentinus edodes, button mushroom(Agaricus bisporus (J. Lange)Sing), oyster mushroom(Pleurotus ostreatus), Auricularia auricula(Hook) Underw, Hericium erinaceum, Hydnum erinaceum Fr., Flammulina velutipes.COPYRIGHT KIPO 2013본 발명은 황금(Au)성분이 함유된 버섯의 배지 조성물 및 그의 재배 방법에 관한 것으로서, 더욱 상세하게는 새송이버섯, 표고버섯, 양송이버섯, 느타리버섯, 목이버섯, 노루궁댕이버섯, 팽이버섯 등 담자균류(Basidiomycets)에 속하는 미생물의 모든 버섯류 에 통합배지로서 분쇄한 볏집(또는 참나무톱밥,미루나무톱밥, 미송나무톱밥, 포플러나무톱밥), 콩비지(또는 대두피, 대두박, 소맥피, 옥수수분말, 밀기울), 미강, 닭똥분말, 수산화칼슘분말, 석고분말, 목화솜, 칡뿌리분말, 미세조류(microalgae)분말, 커피찌꺼기분말, 한약제찌꺼기에 약 20-35나노의 콜로이달 상태의 1,000ppm농도 황금(Au) 이온액을 배지 전체 중량비에 1-10ppm을 물에 첨가하여 분산배합하고 섭씨 100-120℃에서 5-10시간 스팀살균처리 후 섭씨 20℃로 냉각한 황금(Au)이온 혼합배지를 플라스틱