The present invention provides a method for cultivating a mushroom using a sawdust medium. The method for cultivating a mushroom using a sawdust medium comprises: a step of manufacturing a sawdust medium for mushroom cultivation comprising oak tree sawdust, calcium carbonate, wheat bran, cottonseed bran, brown sugar, water, nano-detoxification sulfur, and anthocyan； a step of inserting the sawdust medium into a cultivation container, sealing the cultivation container, and then heating and cooling the cultivation container for sterilization； a step of injecting mushroom spawn into the cooled sawdust medium； a step of cultivating the mushroom spawn in the dark to cultivate a mycelium； a step of cultivating the mushroom mycelium which has undergone dark cultivation in a lit environment to allow browning； and a step of inserting the cultivation container containing the browned mushroom mycelium into a cultivation facility to grow the mushroom mycelium into a mushroom.본 발명은 참나무톱밥, 탄산칼슘, 소맥피, 면실피, 흑설탕, 물, 나노제독유황 및 안토시안을 포함하는 버섯재배용 톱밥배지를 제조하는 단계； 상기 톱밥배지를 배양용기에 담고 밀봉한 후, 살균을 위해 가열하고 냉각하는 단계；.상기 냉각된 톱밥배지에 버섯 종균을 접종하는 단계； 상기 버섯 종균을 암배양을 통해 균사체를 배양하는 단계； 상기 암배양을 거친 버섯 균사체를 명배양하여 갈변화를 진행시키는 단계； 및 상기 갈변화가 완료된 버섯균사체를 포함하는 배양용기를 재배사에 입사하여 버섯으로 배양하는 단계를 포함하는 톱밥배지를 이용한 버섯의 재배방법을 제공한다.
