The present invention relates to a method for preparing vinegar added with leaves of akebia quinata having antitumor, anti-oxidative and antibacterial activities, manufactured vinegar added with leaves of akebia quinata, and a functional food composition containing the same and, more specifically, to a method for preparing vinegar added with leaves of akebia quinata having antitumor, anti-oxidative and antibacterial activities, manufactured vinegar added with leaves of akebia quinata, and a functional food composition containing the same, which assure that the biological activity of the leaves of the akebia quinata has excellent utilization as the vinegar and a functional material of other foods by making certain that the vinegar manufactured by adding young leaves of the akebia quinata has excellent efficacy in functionality, anti-oxidative activity, + radical inhibitory activity, antitumor activity and antibacterial activity.COPYRIGHT KIPO 2016본 발명은 항산화, 항암 및 항균 활성을 갖는 으름잎식초의 제조방법, 제조된 으름잎식초 및 이를 함유하는 기능성 식품조성물에 관한 것으로, 보다 상세하게는 으름의 어린잎을 첨가하여 제조한 식초의 우수한 관능도, 항산화 활성효과, ABTS.+ 라디칼 저해활성, 항암활성 및 항균활성 효능을 확인함으로써 으름잎의 생리활성이 식초 및 기타 식품의 기능성소재로서 활용도가 우수하다는 것을 입증하는 항산화, 항암 및 항균 활성을 갖는 으름잎식초의 제조방법, 제조된 으름잎식초 및 이를 함유하는 기능성 식품조성물에 관한 것이다.