The present invention relates to a method for preparing a deodorant using autotrophs, a deodorant prepared by the method, and deodorization effects using the same. The method for preparing a deodorant using autotrophs according to the present invention prepares a deodorant which is harmless for human body and has deodorization effects and comprises the steps of: culturing autotrophs in order to maintain and obtain optimal population size； sterilizing and purifying the cultured autotrophs to be harmless for water quality, atmosphere, and human body； and collecting the purified autotrophs. Therefore, the deodorant prepared by the preparation method using the autotrophs has, as shown in experimental test data, outstanding effects of directly deodorizing ammonia, trimethylamine, formaldehyde, and hydrogen sulfide and the likes which are generated in daily life. The deodorant has effects of removing foul smell by directly decomposing or converting odor sources, not by indirect neutralizing effects. The present invention provides the deodorant which is harmless for human body, water quality and atmosphere through the sterilization (S3) and purification processes (S4) which are characterizations of the present invention so that a user can directly spray the deodorant in daily life. [Reference numerals] (S1) Screen microorganisms；(S2-1) Culture microorganisms；(S2-2) Mix purified water；(S3-1) Sterilize at -22°；C；(S3-2) Sterilize at 99°；C；(S3-3) Sterilize in a high temperature chamber at 140°；C；(S4) Purify microorganisms；(S5) Harvest microorganisms본 발명은 독립영양미생물(Autotrophs)을 이용한 탈취제의 제조방법 및 이를 통해 제조된 탈취제, 이를 이용한 악취제거 효과에 관한 것이다.이 발명에 따른 독립영양미생물(Autotrophs)을 이용한 탈취제의 제조방법은 독립영양미생물을 적절한 개체수로 유지 및 확보하기 위한 배양단계와, 그 배양된 독립영양미생물(Autotrophs)을 수질이나 대기 및 인체에 무해하게 하기 위한 멸균 및 정제단계와, 정제된 독립영양미생물(Autotrophs)을 수확하는 수확단계를 거쳐 인체에 무해하지만 탈취효과를 갖는 탈취제를 제조하는 것을 특징으로 한다.따라서, 이 독립영양미생물(Autotrophs)을 이용한 제조방법에 의해 제조된 탈취제는 실험데이터에서 제시하는 바와 같이 직접 일상생활에서 발생되는 악취인 암모니아, 트리메틸아민, 포름알데히드 및 황화
