Federalnoe gosudarstvennoe byudzhetnoe obrazovatelnoe uchrezhdenie vysshego obrazovaniya "Moskovskij gosudarstvennyj universitet imeni M.V. Lomonosova" (MGU)
发明人:
Gote Sergej Vladimirovich,Готье Сергей Владимирович,Shagidulin Murat Yunusovich,Шагидулин Мурат Юнусович,Onishchenko Nina Andreevna,Онищенко Нина Андреевна,Nikolskaya Alla Olegovna,Никольская Алла Оле
申请号：
RU2016108045
公开号：
RU0002618989C1
申请日:
2016.03.09
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention refers to the medicine, cellular transplantology, and hepatology. An implantation of a cell-engineering structure (CES) is performed into the liver parenchyma with the further prescription of anticoagulants and antiaggregants of a preventive dose. Whereupon, first, a matrix from a decellularized donor liver of a mammal in the physiological solution buffered with phosphates and having the following composition: 138 mM NaCl, 2.67 mM KCl, 1.47 mM KH2PO4, 8.1 mM Na2HPO4, distilled water under 1 l and containing fibronectin and laminin in the amount of 10 µg/ml, with pH 7.4, is incubated during 8-12 hours at 4°C. The volume ratio of the matrix and physiological solution is 1:1. Then, a co-culture of the freshly-isolated allogenic liver cells and preliminarily cultured allogenic or autologous mesenchymal stem cells of the bone marrow with the ratio of the bone marrow cells to the liver cells from 1:1 to 1:10 is conducted on the matrix during 2-4 days providing the attachment of the cells in the amount of 2106-15106 per 1 cm3 of the matrix. The total matrix volume with the attached cells is at least 0.05 cm3. The CES is obtained before the transplantation by mixing the matrix and the cells attached to it with the biopolymer heterogenic collagen-containing hydrogel with the volume ratio of 3:1. In a separate case, the matrix is obtained from a decellularized donor liver of a human. The matrix may have particle sizes from 200 to 700 mcµm, pore sizes of no more than 50 mcm, and a summarized porosity of 70-85%. Spherogel may be used as a heterogenic biocompatible biodegradable gel.EFFECT: method of improving of the treatment of the liver failure by means of actuation of bilateral interactions between the implanted CESs as a ready hepatolike structures and the parenchyma of a damaged liver of a recipient with the quickened integration of them into the damaged liver.4 cl, 5 dwgИзобретение относится к медицине, клеточной трансплантологии, гепа
