GOSUDARSTVENNOE UCHREZHDENIE ZDRAVOOKHRANENIJA NAUCHNO-ISSLEDOVATELSKIJ INSTITUT SKOROJ POMOSHCHI IMENI N.V. SKLIFOSOVSKOGO DEPARTAMENTA ZDRAVOOKHRANENIJA G. MOSKVY
发明人:
KHUBUTIJA MOGELI SHALVOVICH,Хубутия Могели Шалвович (RU),KHVATOV VALERIJ BORISOVICH,Хватов Валерий Борисович (RU),GULJAEV VLADIMIR ALEKSEEVICH,Гуляев Владимир Алексеевич (RU),ZHURAVEL SERGEJ VLADIMIRO
申请号：
RU2011111228/14
公开号：
RU0002452519C1
申请日:
2011.03.25
申请国别(地区):
RU
年份:
2012
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention refers to medicine, namely transplantology, anesthesiology, resuscitation and transfusion medicine, and is applicable for the purpose of intraoperative and early postoperative compensation of acute profuse blood loss accompanying transplantation. That is ensured by preoperative recovery of multi-organ brain-death donor organs combined with blood banking from an inferior vena cava system. It is preceded by the intravenous introduction of heparin 25000-50000 units to the donor according to body weight. An aorta is cannulated through one of iliac arteries. Two ligatures are introduced under an infrarenal inferior vena cava with a vessel notched in between. A blood sample cannula is introduced in a distal direction through a vein orifice, and exhaustion 5-10 mm Hg makes blood arriving the distal inferior vena cava into the sterile container of a continuous autotransfusion system. In a proximal direction, a silicone tube is introduced in the inferior vena cava to remove mixed blood and preserving agent. Loss of systolic blood pressure 80 mm Hg and lower requires ligaturing the aorta above an arterial trunk. Through the aortic cannula, the preserving agent custadiol for cold perfusion is introduced. Another iliac artery is ligatured. Venous blood collected in the sterile container, exposed to cell separated in a sparing mode - 3 washing cycles at erythromass rate 30-70 ml/min. It is followed by cell concentration to packed cell volume 65-75 vl % with using the apparatus Cell Saver the prepared product is marked as a cell blood component of donor organs (CBCDO). The prepared CBCDO volume within 500 to 1500 ml contains: 3,9-12,0×1012 red blood cells or 1.7-5.4 standard red blood cell doses 57-171×109 platelets or 0.2-0.6 standard therapeutic platelet concentrate doses 48-81×106 CD34+ haematopoietic stem cells and 100-264×106 CD105+ multipotent mesenchymal stromal cells. The CBCDO is shifted with a reflux pump into a blood transfusion con
