FEDERALNOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE "FEDERALNYJ NAUCHNYJ TSENTR TRANSPLANTOLOGII I ISKUSSTVENNYKH ORGANOV IMENI AKADEMIKA V.I. SHUMAKOVA" MINISTERSTVA ZDRAVOOKHRANENIJA ROSSIJSKOJ FEDE
发明人:
GOTE SERGEJ VLADIMIROVICH,Готье Сергей Владимирович,KRASHENINNIKOV MIKHAIL EVGENEVICH,Крашенинников Михаил Евгеньевич,ONISHCHENKO NINA ANDREEVNA,Онищенко Нина Андреевна,SHAGIDULIN MURAT JUNUSOVICH,Шаг
申请号：
RU2013152894/15
公开号：
RU0002539918C1
申请日:
2013.11.29
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
FIELD: medicine.SUBSTANCE: tissue-specific matrix is obtained by performing a perfusion washing and a decellularisation of a parenchymal organ. Herewith 60-120 minutes before the perfusion washing of the donor organ, measures to prevent intravascular blood cell aggregation and microcirculation disturbances are taken and involve the intramuscular or intravenous administration of a disaggregant or disaggregants (heparin and trental) into a donor. That is followed by an organ perfusion by administering into its vascular bed phosphate-buffered normal saline of the following composition: 138 mM NaCl, 2.67 mM KCl, 1.47 mM KH2PO4, 8.1 mM Na2HPO4, distilled water up to 1l and containing 1% serum albumin and 10-15% glycerol or 10-15% dimethyl sulphoxide with pH 7.4 in an amount equal to a double amount of the vascular bed of the organ at a perfusion pressure of 100-120 mm Hg in its arterial system. Thereafter, the decellularised organ is ground to a particle size of 0.5mm to 4mm the ground fragments are portioned in an amount of 5-10g, and each portion is frozen by immersing into liquid nitrogen gas for 5-10 minutes. The frozen portions are re-ground to a particle size of no more than 600 mcm then each portion is de-frozen by re-suspending in phosphate-buffered normal saline 30-70ml of the following composition containing 138 mM NaCl, 2.67 mM KCl, 1.47 mM KH2PO4, 8.1 mM Na2HPO4, distilled water up to 1l , with pH 7.4, at a room temperature. The prepared suspension is cleaned from the particles of less than 200mcm the prepared fraction is lyophilised and sterilised to prepare the target matrix samples.EFFECT: using the invention enables providing the more complete decellularisation procedure ensured by preventing the disturbed microcirculation in the donor organ, providing higher density and uniformity of the re-cellularisation of the entire matrix and easier control thereof by increasing the matrix adhesive surface areas for the contact to inoculating cells as prepared in th
