The present invention relates to a method for increasing the content of amino acids by using microorganisms. The present invention relates to a manufacturing method of health supplementary food by washing, drying, pulverizing and powderizing at least one raw material selected from Rehmannia root, ginseng, Poria cocos Wolf, corni, dodder, Liriope platyphylla, Asparagus cochinchinensis (Lour.) Merr., Rubus coreanus, maca, sea cucumber, Angelica gigas, agilawoods and deer antlers； sterilizing under conditions of 121°；C and 1-2 atmosphere for 10-30 minutes； inoculating with cultured liquid in which microorganisms are cultivated at 40-60°；C and pH 6.0-7.5； fermenting the mixture at 40-60°；C for 4-7 days by a solid phase fermentation； drying the fermened mixture； and kneading with white wheat. The present invention provides a manufacturing method of health supplementary food by extracting the raw materials with hot water at 90-121°；C for 4-8 hours； culturing microorganisms in a sterilized liquid medium where microorganisms grow well to manufacture microorganism cultivation liquid； mixing the microorganism cultivation liquid with 5-10 weight% of the hot water extract and fermenting at 40-60°；C and pH 6.0-7.5 for 4-7 days to manufacture liquid ferment； powderizing the liquid fermentation by a method selected from hot wind drying, freeze-drying, and supercritical drying； and forming the powder in a form of powder, granules, tablets, capsules, jelly, cream or porridge. [Reference numerals] (S11) Mix raw materials； (S12,S15-1) Sterilization； (S13) Microorganism culture； (S14) Mixed culture； (S15-2) Probiotics； (S16) Manufacture health supplementary food본 발명은 미생물을 이용하여 아미노산 함량을 증가시키는 방법에 관한 것으로, 생지황, 인삼, 복령, 산수유, 토사자, 맥문동, 천문동, 복분자, 마카, 해삼, 당귀, 침향, 녹용 중 적어도 하나 이상이 선택되는 원료를 수세 및 건조하여 분쇄하거나 분말하여 121℃에서 1 내지 2기압의 조건하에서 10 내지 30분간 멸균한 후 미생물을 40 내지 60℃, pH 6.0 내지 7.5 조건에서 배양한 배양액을 접종하고 4 내지 7일간, 40 내지 60℃ 조건에서 고상발효하며, 상기 고상발효한 것을 건조하여 백밀과 반죽한 건강보조식품의 제조방법과；상기 원료를 90
