Provided are an insulin-lipid complex, a preparation method therefor, and a preparation therefor. The insulin-lipid complex is prepared by compounding insulin and a lipid material in organic solvent system containing a low boiling point acid, and drying. The mass ratio of insulin to the lipid material is1:3ˆ¼1:20. An oil solution of the insulin-lipid complex and vesicles containing insulin are further provided.