The present invention relates to processes for separating wheat flour into two or more fractions including a gluten fraction and a starch fraction, comprising the steps of: a) mixing wheat flour and water； b) adding one or more polypeptide (s) having GH8 xylanase activity； c) incubating the mixture for a predefined period of time； d) separating the mixture into two or more fractions including a gluten rich fraction and a starch rich fraction； and e) recovering the two or more fractions including a gluten rich fraction and a starch rich fraction.
