BUAN-GUN(MANAGEMENT；BUAN-GUN AGRICULTURAL DEVELOPMENT AND TECHNOLOGY CENTER);부안군(관리부서: 부안군 농업기술센터);전북대학교산학협력단;INDUSTRIAL COOPERATION FOUNDATION CHONBUK NATIONAL UNIVERSITY
The present invention relates to a preparation method of silkworm extract having higher content of deoxynojirimycin and arginine than a conventional extraction method, and more specifically, to a preparation method of silkworm extract having deoxynojirimycin and arginine, which comprises: a step of drying live silkworms and then grinding and pulverizing the dried silkworms to prepare silkworm powder； a first extraction step of adding 95% ethanol aqueous solution to the silkworm powder to make solvent extraction, filtering the solution to separate precipitate and an extract, and then obtaining the extract to prepare an ethanol extract； a second extraction step of adding water to the precipitate separated in the first extraction step to make solvent extraction, filtering the solution to separate precipitate and an extract, and obtaining the extract to prepare a water extract； and an extract preparing step of mixing the ethanol extract and the water extract. The silkworm extract prepared by the preparation method of the present invention has high content of deoxynojirimycin which was conventionally appreciated to have excellent antidiabetic activity, and also high content of arginine. Accordingly, a large amount of amino acids are determined to be contained； functional ingredients of silkworms will be included at maximum； and extraction time can be minimized to minimize extraction costs and time, thereby improving economic effects of an extraction method. Consequently, aside from sericultural industry, utilization of silkworms can extend to food industry or the like, thereby contributing to rising income of silkworm farms and activated local economy, such that huge industrial effects thereof are expected.본 발명은 기존 추출방법에 비하여, 데옥시노지리마이신 및 아르기닌의 함량이 증가된 누에 추출물 제조방법, 구체적으로 생누에를 건조한 후, 분쇄하여 분말화하여 누에 분말을 제조하는 단계； 상기 누에 분말에 95% 에탄올 수용액을 첨가하고 용매추출한 후, 여과하여 침전물 및 추출액을 분리하고, 추출액을 수득하여 에탄올 추출액을 제조하는 제1 추출단계； 제1 추출단계에서 분리된 침전물에 물을 첨가하고 용매추출한 후, 여과하여 침전물 및 추출액을 분리하고, 추출액을 수득하여 물 추출
