JULIA BINTI IBRAHIM,KUA SHWU FUN,NORHAFIZI BIN HASHIM,KHAIRUL IZWAN BIN NAN,CHRISTOPHER OOI KUOK WENG,HIRZUN MOHD YUSOF,HARIKRISHNA KULAVEERASINGAM
申请号：
MYUI 2013700837
公开号：
MY165428A
申请日:
2013.05.21
申请国别(地区):
MY
年份:
2018
代理人:
摘要：
THE PRESENT DISCLOSURE RELATES GENERALLY TO A PROCESS FOR EXTRACTING PROTEIN FROM PALM KERNEL CAKE (PKC) AND PRODUCING HIGH PROTEIN CONTENT PKC COMPRISING: (I) MIXING PKC WITH AN EFFECTIVE AMOUNT OF ONE OR MORE HEMICELLULOSE DEGRADING ENZYMES SUCH AS MANNANSE AND PECTINASE IN AN AQUEOUS SOLUTION IN A RATIO OF PKC TO SOLUTION (W/V) FROM ABOUT 1:2 TO ABOUT 1:5 TO OBTAIN A PKC HYDROLYSATE AND HYDROLYSED PKC (II) SEPARATING THE PKC HYDROLYSATE FROM THE HYDROLYSED PKC VIA DECANTATION, FILTRATION OR CENTRIFUGATION (III) PRECIPITATING PROTEIN FROM SAID HYDROLYSATE BY THE ADDITION OF AN ANTICHAOTROPIC SALT IN AN AMOUNT IN THE RANGE OF 10-90W/V% SELECTED FROM AMMONIUM SULPHATE, SODIUM SULPHATE AND SODIUM CHLORIDE AND (IV) DRYING THE HYDROLYSED PKC, WHEREIN THE PKC IS EXPELLER PRESSED PKC OR SOLVENT EXTRACTED PKC WHEREIN THE HYDROLYSIS STEP IS PERFORMED AT A TEMPERATURE IN THE RANGE OF ABOUT 20-80°C, AT A PH IN THE RANGE OF ABOUT 3-8 AND FOR A PERIOD OF TIME IN THE RANGE OF ABOUT 12-168 HOURS WHEREIN THE SEPARATED PKC HYDROLYSATE IS SUBJECTED TO A CLARIFICATION STEP TO AFFORD AN-AQUEOUS PHASE AND AN OIL-CONTAINING PHASE AND THE AQUEOUS PHASE IS SUBJECTED TO CROSSFLOW FILTRATION TO AFFORD A PROTEIN CONCENTRATE WHEREIN THE PROTEINS ARE PRECIPITATED FROM THE CONCENTRATE BY THE ADDITION OF ANTICHAOTROPIC ORGANIC SOLVENT (ETHANOL) IN THE RANGE OF ABOUT 50-90 V/V% WHEREIN THE PRECIPITATED PROTEIN IS CENTRIFUGED, WASHED AND FREEZE DRIED AND WHEREIN THE HYDROLYSED PKC HAS A PROTEIN CONTENT IN THE RANGE OF 20%-30%. MOST ILLUSTRATIVE DRAWING: FIGURE 1
