A sterilization tray is disclosed. The sterilization tray comprises a basin and a platter including a depression and a port disposed through a terminal end of the depression. The basin and the platter define a first chamber and the port defines a passage into the first chamber. An adapter may be connected to the port to facilitate connection of an endoscope thereto. The tray and the endoscope may be disposed in a vacuum chamber of a sterilizer and subject to a sterilization cycle. A pressure differential may be created between the vacuum chamber and the first chamber. A sterilant may be drawn through the insertion tube and into the first chamber. The sterilant may also be drawn through the insertion tube and into the vacuum chamber.本發明公開了一種滅菌托盤。該滅菌托盤包含:一盆體及一淺盤,該淺盤包括一凹陷部及穿過該凹陷部的一終端設置的一接口。該盆體及該淺盤界定一第一室,而該接口界定進入該第一室的一通道。一配接器可連接到該接口以有利於一內視鏡與其的連接。該托盤及該內視鏡可設置在一滅菌器的一真空室中並經受一滅菌循環。該真空室與該第一室之間可形成壓力差。一滅菌劑可經抽吸通過該插入管並進入該第一室。該滅菌劑亦可經抽吸通過該插入管並進入該真空室。