A sterilization tray is disclosed. The sterilization tray comprises a basin and a platter including a depression and a port disposed through a terminal end of the depression. The basin and the platter define a chamber and the port defines a passage into the chamber. An adapter may be connected to the port to facilitate connection of an endoscope thereto. The tray and the endoscope may be disposed in a vacuum chamber of a sterilizer and subject to a sterilization cycle. A pressure differential may be created between the vacuum chamber and the first chamber. A sterilant may be drawn through the insertion tube and into the first chamber. The sterilant may also be drawn through the insertion tube and into the vacuum chamber.
