A METHOD FOR MANUFACTURING A PEPTIDE OF PELODISCUS SINENSIS, THE PEPTIDE OF PELODISCUS SINENSIS, AND THE USE OF THE PEPTIDE OF PELODISCUS SINENSIS BEING MANUFACTURING A DRUG FOR REDUCING BLOOD PRESSURE THEREOF
NATIONAL PINGTUNG UNIVERSITY OF SCIENCE & TECHNOLOGY
发明人:
HSU, JUE LIANG,徐睿良,徐睿良,CHANG, CHI I,张志益,張誌益,HUANG, TZOU CHI,黄卓治,黃卓治
申请号：
TW102140771
公开号：
TW201518508A
申请日:
2013.11.08
申请国别(地区):
TW
年份:
2015
代理人:
摘要：
A method with simple steps for manufacturing a peptide of Pelodiscus sinensis with activity of inhibiting angiotensin I-converting enzyme is disclosed. The method comprises: providing an egg white solution dissolved with a buffer, providing thermolysin with activity being 50-100 U/mg, and mixing the thermolysin with the egg white in a E/S weight ratio of 1/50-1/900, followed by hydrolysis the egg white solution at pH 6-9, 40-60℃ for 1-10 hours to obtain a hydrolysate containing the peptide of Pelodiscus sinensis with a sequence set forth in SEQ ID NO: 1.一種鱉蛋胜肽製備方法,係以簡單的步驟獲得具有降低血管增壓素轉換酶活性之鱉蛋胜肽,其包含:提供一鱉蛋蛋白溶液,係將一鱉蛋蛋白溶於一緩衝液;提供一嗜熱菌蛋白酶,該嗜熱菌蛋白酶活性單位為50~100U/mg;以酵素受質比為1/50~1/900之重量比例混合該嗜熱菌蛋白酶及該鱉蛋蛋白,於酸鹼值為pH 6~9及溫度50~70℃之條件中進行水解反應1~10小時,以得到一水解溶液,該水解溶液係含有鱉蛋胜肽,且該鱉蛋胜肽係包含如SED ID NO:1所示序列。
