UNIVERSITE CLAUDE BERNARD LYON 1;INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE;CENTRE LEON BERARD;NEOLYS DIAGNOSTICS;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
发明人:
NICOLAS FORAY,ADELINE GRANZOTTO,CLEMENT DEVIC,MELANIE FERLAZZO,LARRY BODGI,SANDRINE PEREIRA
申请号：
FR1563362
公开号：
FR3045071A1
申请日:
2015.12.28
申请国别(地区):
FR
年份:
2017
代理人:
摘要：
A method for characterizing the radiosensitivity of a cell sample of a patient to ionizing radiation, said cell sample having been obtained from cells taken from a patient, wherein the method comprises: (a) isolating target nucleated cells cell sample； (b) irradiating said cell sample before or after step (a) with an absorbed dose D of ionizing radiation； (c) recovering cytoplasmic and nuclear fractions from the cell sample； (d) determining, on the cytoplasmic and / or nuclear fractions of said cell sample, either: (d1) the amount of substrate phosphorylated by the activated ATM protein at at least one observation time t between t1 times 1, 2 and t3 , preferably at least one observation time t2 after irradiation with an absorbed dose D, (d2) the kinase activity by quantifying pATM phosphorylated at at least one observation time t selected from t = t1, t2； (e) determining at least one parameter selected from the group consisting of: the grade of severity of the post-radiotherapy tissue reaction of the patient according to the CTCAE classification, using at least the average number pATMmax nuc (t)； radiosensitivity of the cell sample using at least the average number pATM maxnuc (t)； and where t1 is a fixed value which represents the time at which the number of recognized double-strand breaks (DSBs) peaks in control cells from radiation-resistant patients； • t2 is a fixed value representing the time at which approximately 50% of CBDs are repaired in control fibroblasts from radiation-resistant patients and the time after which 30% of CBDs are repaired in control lymphocytes or lymphoblasts from radiation-resistant patients ； T3 is a fixed value which represents z times t2, where z is between 1.1 and 8, and preferably between 1, 2 and 6. Procédé pour caractériser la radiosensibilité d'un échantillon cellulaire d'un patient envers un rayonnement ionisant, ledit échantillon cellulaire ayant été obtenu à partir de cellules prélevées sur un patient, dans lequel pr
