The present invention relates to a preparation method for a therapeutic agent of a bead-type chondrocyte and, more specifically, to: a preparation method for a therapeutic agent of a bead-type chondrocyte without a support, comprising the steps of: a) dispensing chondrocytes and/or cells having chondrogenic differentiation capability in a 96-well deep well plate having a V-shaped bottom； b) centrifuging the plate； c) three-dimensionally culturing the plate in an incubator； and d) recovering pellets from each well, thereby being capable of easily and stably preparing cartilage tissues of a uniform quality, in large quantities； and a therapeutic agent of a bead-type chondrocyte without a support, prepared by the method, having effectiveness to repair damage by simply being implanted into cartilage damage by injection.
