The present invention relates to a method for screening probiotic bacteria having anti-aging activity using Caenorhabditis elegans, and more particularly, in screening probiotic bacteria exhibiting an extended life span efficacy, to a method for screening probiotic bacteria having anti-aging activity in vivo quickly and simply by using Caenorhabditis elegans which is a novel experimental animal, and the present invention provides anti-aging health functional food containing, as an active ingredient, the probiotic bacteria having the anti-aging activity screened from the screening method.COPYRIGHT KIPO 2017