PROBLEM TO BE SOLVED: To provide analysis of an amino acid sequence and a base sequence of a specific enzyme related to a biosynthetic pathway of polyisoprenoid in Solidago altissima which can be a stable supply source of natural rubber other than a mature tree of Hevea brasiliensis.SOLUTION: Protein of the present invention is any of A1 to A3: (A1) protein composed of a specific amino acid sequence (A2) protein which is composed of the specific amino acid sequence in which one or plural amino acids are substituted, deleted, inserted, and/or added, and has enzyme activity catalyzing a reaction in which isopentenyl diphosphate and dimethylallyl diphosphate are used as substrates, or a reaction in which isopentenyl diphosphate and geranyl diphosphate are used as substrates (A3) protein which is composed of an amino acid sequence having a sequence identity of 80% or more with the specific amino acid sequence, and has enzyme activity catalyzing a reaction in which isopentenyl diphosphate and dimethylallyl diphosphate are used as substrates, or a reaction in which isopentenyl diphosphate and geranyl diphosphate are used as substrates.COPYRIGHT: (C)2015,JPO&INPIT【課題】パラゴムノキの成木以外の安定的な天然ゴムの供給源となり得るセイタカアワダチソウのポリイソプレノイド生合成経路に関する特定の酵素のアミノ酸配列及び塩基配列の解析の提供。【解決手段】A1~A3のいずれかに記載の蛋白質。A1:特定のアミノ酸配列からなる蛋白質、A2:特定のアミノ酸配列において、1若しくは複数個のアミノ酸の置換、欠失、挿入、及び/又は付加を含む配列からなり、イソペンテニル二リン酸とジメチルアリル二リン酸とを基質とする反応、又はイソペンテニル二リン酸とゲラニル二リン酸とを基質とする反応を触媒する酵素活性を有する蛋白質、A3:特定のアミノ酸配列と80%以上の配列同一性を有するアミノ酸配列からなり、イソペンテニル二リン酸とジメチルアリル二リン酸とを基質とする反応、又はイソペンテニル二リン酸とゲラニル二リン酸とを基質とする反応を触媒する酵素活性を有する蛋白質【選択図】なし
