PROBLEM TO BE SOLVED: To provide an enterocyte activating composition that has the excellent effect to activate enterocytes and promote the proliferation thereof and is suitable for making the intestine sound.SOLUTION: An enterocyte activating composition comprises leaves of barley or the like or their processed products, and further comprises water-soluble dietary fiber such as indigestible dextrin, oligosaccharide such as isomaltooligosaccharide, and lactic acid bacteria.