YOON, WEON JONG,윤원종,HAM, YOUNG MIN,함영민,JUNG, YONG HWAN,정용환,PARK, SOO YEONG,박수영,OH, DAE JU,오대주
申请号：
KR1020110139005
公开号：
KR1020130071666A
申请日:
2011.12.21
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: An anti-inflammatory composition using vladinol F isolated from F. erecta var. sieboldii is provided to suppress generation of NO, PGE2 (prostaglandin), and inflammatory cytokines (TNF-alpha, IL-1beta, and IL-6).CONSTITUTION: An anti-inflammatory composition contains vladinol F as an active ingredient. A method for isolating vladinol F from F. erecta var. sieboldii comprises the steps of: dipping F. erecta var. sieboldii leaves, stems, or a mixture thereof in a mixture solvent of ethanol and water and preparing a power extract suspending the extract in distilled water, and sequentially fractioning to obtain a dichloromethane fraction coating a celite surface with the fraction and performing column chromatography using hexane, dichloromethane, ethyl acetate, and methanol to obtain 4 purified materials performing normal phase column chromatography of a second purified material using hexane:ethyl acetate:methanol (1:2:0.2, v/v) to obtain total 20 purified materials performing normal phase silicagel column chromatography of 16th purified material among 20 purified materials using chloroform:methanol (10:1, v/v) and preparing total 18 purified materials and collecting 9th purified material among 18 purified materials.COPYRIGHT KIPO 2013[Reference numerals] (AA) MC fraction of a F. erecta var. sieboldii extract (11.2g) (BB) Column chromatography (CC) Normal phase silica gel (5cm×60cm) (DD) Normal phase silica gel (2.0cm×50cm)본 발명은 좁은잎천선과로부터 분리한 활성물질인 블라디놀 F를 이용한 항염증성 조성물을 개시한다. 상기 활성물질인 블라디놀 F는 NO 생성 억제 활성, PGE2 생성 억제 활성 및 염증성 사이토카인(TNF-α, IL-1β 및 IL-6)의 생성 억제 활성을 갖는다.
