Chen, Fuqiang,Davis, Gregory D.,Kang, Qiaohua,Knight, Scott W.
申请号:
AU2017204031
公开号:
AU2017204031A1
申请日:
2017.06.15
申请国别(地区):
AU
年份:
2017
代理人:
摘要:
#$%^&*AU2017204031A120170706.pdf#####ABSTRACT The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.