Chen, Fuqiang,Davis, Gregory D.,Kang, Qiaohua,Knight, Scott W.
申请号：
AU2020230243
公开号：
AU2020230243A1
申请日:
2020.09.08
申请国别(地区):
AU
年份:
2020
代理人:
摘要：
#$%^&*AU2020230243A120201001.pdf#####ABSTRACT The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.
