CHEN, FUQIANG,DAVIS, GREGORY D.,KANG, QIAOHUA,KNIGHT, SCOTT W.
申请号：
SG11201503824S
公开号：
SG11201503824SA
申请日:
2013.12.05
申请国别(地区):
SG
年份:
2015
代理人:
摘要：
The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.
