Chen, Fuqiang,Davis, Gregory D.,Kang, Qiaohua,Knight, Scott W.
申请号：
AU2018229489
公开号：
AU2018229489B2
申请日:
2018.09.13
申请国别(地区):
AU
年份:
2018
代理人:
摘要：
#$%^&*AU2018229489B220181206.pdf#####ABSTRACT The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.WO 2014/089290 1/7 PCT/US2013/073307 A Cas,- L ......................... ....... ............. ......................... ....... ............. ........................................... ........................................... ........................................... ........................................... ........................................... ........................................... ........................................... ........................................... ........................................... ........................................... ................. ................. iffiff. i i I ....... Target DNA ............................................ ............................................ ........................................... ............................................ ........................................... ............................................ ........................................... ............................................ B ............ ............. ............... ............................................. - -............... ............................................. - -............... ...................
