The present disclosure relates to multi-specific protein molecules. In particular, the invention relates to a multi-specific antibody with a new structural form. The multi-specific antibody can combine with CD3 and other tumor related antigens at the same time, combine with tumor related antigen expression cells, and activate CD3 positive T cells, so as to promote the specific killing of T cells to tumor cells expressing tumor related antigens. At the same time, the present disclosure also provides the preparation and application of multi-specific antibodies.本公開涉及多特異性蛋白分子。具體涉及一種具有新的結構形式多特異性抗體。多特異性抗體能夠同時與CD3和其他腫瘤相關抗原相結合，結合腫瘤相關抗原表達細胞的同時，結合並激活CD3陽性的T細胞，進而促進T細胞對表達腫瘤相關抗原的腫瘤細胞特異性的殺傷。同時，本公開還提供多特異性抗體的製備及應用。
