H. LEE MOFFITT CANCER CENTER AND RESEARCH INSTITUTE; INC.
发明人:
Marco DAVILA
申请号：
US16334073
公开号：
US20190211075A1
申请日:
2017.09.19
申请国别(地区):
US
年份:
2019
代理人:
摘要：
Disclosed herein are methods of producing chimeric antigen receptor (CAR) T cells using substrates, such as artificial antigen presenting cells, containing on a surface a a heparin binding domain (HBD), anti-CD3 single chain antibodies, anti-CD28 single chain antibodies (scFv), and optionally anti-41BBL antibodies. Anti-CD3 and Anti-CD28 scFvs bind and activate expanding T cells ex vivo, while the Heparin Binding Domain binds the viral vector, thereby bringing the T cells into close proximity with virus for effective gene transfer. This is a less costly, renewable, modifiable, and efficacious alternative to coated beads and RetroNectin® for gene transfer.
