Public University Corporation Nagoya City University
发明人:
申请号：
EP18799226.8
公开号：
EP3623469A1
申请日:
2018.05.02
申请国别(地区):
EP
年份:
2020
代理人:
摘要：
An object of the present invention is to prepare a functional intestinal organoid from pluripotent stem cells. An intestinal organoid is prepared from pluripotent stem cells, by the following steps (1) to (4): (1) differentiating pluripotent stem cells into endoderm-like cells； (2) differentiating the endoderm-like cells obtained in step (1) into intestinal stem cell-like cells； (3) culturing the intestinal stem cell-like cells obtained in step (2) to form spheroids； and (4) differentiating the spheroids formed in step (3) to form an intestinal organoid, the step including culture in the presence of a MEK1/2 inhibitor, a DNA methylation inhibitor, a TGF-β receptor inhibitor, and a γ-secretase inhibitor, in addition to an epidermal growth factor, a BMP inhibitor, and a Wnt signal activator.
